Test used to detect Lipids

 Test used to detect lipids?

Following tests are used to detect lipids:

(i)    Sudan-III Test: 

Experiment	Observation	Inference
2 ml original solution in test tube + 2 ml water + Few drops of Sudan III + Shake well.	Red stained oil layer separates on surface of water, which remains uncoloured.	Lipid present.

 

(ii)    Emulsion Test:

Experiment	Observation	Inference
2 ml O.S + 2 ml absolute ethanol + Shake well + Equal volume of cold water	Cloudy white suspension is formed.	Lipid present.

 

What are lipids ?

Lipids are the organic compounds made up of carbon, hydrogen and oxygen, are characterized by their solubility in organic solvents such as ether, alcohol and their insolubility in water.