Differentiate between Restriction endonuclease and ligase?


DEFINITIONS

Cloning: 
A technique for developing large numbers of genetically identical cells or organisms is known as cloning.

Recombination:
Formation of a new association of DNA molecules or part of DNA molecules is termed as recombination.

Vector:
A plasmid that carries an inserted pieces of DNA into a host cell in recombinant DNA technology

(OR)

Any DNA molecule, such as plasmid, which serves to carry foreign DNA into host cells, where it may be replicated and expressed.

Gene:
An unit of inheritance is called gene.

Genome:
The total genetic constitution of an organism is known as genome.


Q No.2 Differentiate between restriction endonuclease and ligase?

Restriction Endonuclease (Scissor):
(i) It is the enzyme which can cut a DNA molecule within the strand.
(ii) It is also termed as "scissor".
(iii) "Restriction endonuclease" recognizes specific nucleotide sequence in DNA and then cut both strands in specific manner.

Ligase (Glue):
(i) The enzyme which has ability to seal up the DNA molecule.
(ii) Is is also termed as "glue".
(iii) "Enzyme that creates bonds between the ends of DNA molecules and form a large polynucleotide".

Differentiate between Restriction endonuclease and ligase? Differentiate between Restriction endonuclease and ligase? Reviewed by SaQLaiN HaShMi on 2:04 AM Rating: 5

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